<?xml version="1.0" encoding="UTF-8"?>
<!--REG30PARAB V1.0 (01-06-2026)-->
<xbrli:xbrl xmlns:in-capmkt="http://www.sebi.gov.in/xbrl/2026-04-30/in-capmkt" xmlns:in-capmkt-ent="http://www.sebi.gov.in/xbrl/Regulation_30_Para_B_Of_Part_A_Of_Schedule_III/2026-04-30/in-capmkt/in-capmkt-ent" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:nonnum="http://www.xbrl.org/dtr/type/non-numeric" xmlns:in-capmkt-types="http://www.sebi.gov.in/xbrl/2026-04-30/in-capmkt-types" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:net="http://www.xbrl.org/2009/role/net" xmlns:num="http://www.xbrl.org/dtr/type/numeric" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:negated="http://www.xbrl.org/2009/role/negated" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:xl="http://www.w3.org/1999/xlink"><link:schemaRef xlink:type="simple" xlink:href="in-capmkt-ent-2026-04-30.xsd"/><xbrli:context id="MainI"><xbrli:entity><xbrli:identifier scheme="http://www.sebi.gov.in/in-capmkt/ScripCode">544709</xbrli:identifier></xbrli:entity><xbrli:period><xbrli:instant>2026-09-24</xbrli:instant></xbrli:period></xbrli:context><in-capmkt:NSESymbol contextRef="MainI">GAUDIUMIVF</in-capmkt:NSESymbol><in-capmkt:NameOfTheCompany contextRef="MainI">GAUDIUM IVF AND WOMEN HEALTH LIMITED</in-capmkt:NameOfTheCompany><in-capmkt:ScripCode contextRef="MainI">544709</in-capmkt:ScripCode><in-capmkt:MSEISymbol contextRef="MainI">NOTLISTED</in-capmkt:MSEISymbol><in-capmkt:ISIN contextRef="MainI">INE0P8B01020</in-capmkt:ISIN><in-capmkt:TypeOfEventAsPerRegulation30ParaBOfPartAOfScheduleIII contextRef="MainI">Commencement or postponement (Sub-para 1-Para B)</in-capmkt:TypeOfEventAsPerRegulation30ParaBOfPartAOfScheduleIII><in-capmkt:TypeOfAnnouncementAsPerRegulation30ParaBOfPartAOfScheduleIII contextRef="MainI">New</in-capmkt:TypeOfAnnouncementAsPerRegulation30ParaBOfPartAOfScheduleIII><in-capmkt:WhetherEventOrInformationDisclosedIsAnOutcomeOfTheBoardMeeting contextRef="MainI">false</in-capmkt:WhetherEventOrInformationDisclosedIsAnOutcomeOfTheBoardMeeting><in-capmkt:DateOfOccurrenceOfEventOrInformation contextRef="MainI">2026-09-24</in-capmkt:DateOfOccurrenceOfEventOrInformation><in-capmkt:TimeOfOccurrenceOfEventOrInformation contextRef="MainI">18:00:00</in-capmkt:TimeOfOccurrenceOfEventOrInformation><in-capmkt:DateOfReport contextRef="MainI">2026-09-24</in-capmkt:DateOfReport><in-capmkt:DisclosureForUnitOrDivisionOfTheCompany contextRef="MainI">Unit</in-capmkt:DisclosureForUnitOrDivisionOfTheCompany><in-capmkt:DisclosureForCommencementOfCommercialProductionOrCommercialOperationsOfTheCompany contextRef="MainI">Commercial operations</in-capmkt:DisclosureForCommencementOfCommercialProductionOrCommercialOperationsOfTheCompany><in-capmkt:NameOfUnitOrDivisionOfTheCompany contextRef="MainI">Gaudium IVF – Gurgaon Centre</in-capmkt:NameOfUnitOrDivisionOfTheCompany><in-capmkt:DateOfCommencement contextRef="MainI">2026-09-24</in-capmkt:DateOfCommencement><in-capmkt:DetailsOfCommencement contextRef="MainI">In compliance with Regulation 30 read with Para B of Schedule III of the SEBI (Listing Obligations and Disclosure Requirements) Regulations, 2015 read with the SEBI Master Circular dated January 30, 2026, we hereby notify that, in line with the objects of the Initial Public Offer of the Company, as mentioned in the Prospectus dated February 25, 2026, the Company has commenced operations at the Company’s new IVF Centre/Hub at Gurgaon, Haryana with effect from September 24, 2026. 

The Centre/Hub is designed to provide comprehensive fertility and reproductive healthcare services under one roof, supported by experienced fertility specialists, advanced reproductive medicine, modern medical infrastructure and standardised clinical protocols. The Centre features Gaudium IVF’s proprietary Gaudium Signature Lab, which integrates advanced embryology, AI-powered technologies and internationally benchmarked quality standards to provide an optimal environment for embryo development and support high standards of precision, safety and clinical excellence.</in-capmkt:DetailsOfCommencement><in-capmkt:WhetherPriorIntimationOfDateOfCommencementOfCommercialProductionOrOperationsWasGiven contextRef="MainI">false</in-capmkt:WhetherPriorIntimationOfDateOfCommencementOfCommercialProductionOrOperationsWasGiven></xbrli:xbrl>