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<!--PIT V2.0 (30-04-2026)-->
<xbrli:xbrl xmlns:in-bse-co="http://www.bseindia.com/xbrl/co/2017-09-15/in-bse-co" xmlns:in-bse-co-roles="http://www.bseindia.com/xbrl/co/2017-09-15/in-bse-co-roles" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:nonnum="http://www.xbrl.org/dtr/type/non-numeric" xmlns:in-bse-co-type="http://www.bseindia.com/xbrl/co/2017-09-15/in-bse-co-types" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:net="http://www.xbrl.org/2009/role/net" xmlns:num="http://www.xbrl.org/dtr/type/numeric" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:negated="http://www.xbrl.org/2009/role/negated" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:xl="http://www.xbrl.org/2003/XLink"><link:schemaRef xlink:type="simple" xlink:href="in-bse-co-2017-09-15.xsd"/><xbrli:context id="MainI"><xbrli:entity><xbrli:identifier scheme="http://www.bseindia.com/bse-cg/ScripCode">532321</xbrli:identifier></xbrli:entity><xbrli:period><xbrli:instant>2026-08-24</xbrli:instant></xbrli:period></xbrli:context><xbrli:context id="Disclosure1"><xbrli:entity><xbrli:identifier scheme="http://www.bseindia.com/bse-cg/ScripCode">532321</xbrli:identifier></xbrli:entity><xbrli:period><xbrli:instant>2026-08-24</xbrli:instant></xbrli:period><xbrli:scenario><xbrldi:typedMember dimension="in-bse-co:ChangeInHoldingOfSecuritiesOfPromotersAxis"><in-bse-co:ChangeInHoldingOfSecuritiesOfPromotersDomain>Disclosure1</in-bse-co:ChangeInHoldingOfSecuritiesOfPromotersDomain></xbrldi:typedMember></xbrli:scenario></xbrli:context><xbrli:unit id="INR"><xbrli:measure>iso4217:INR</xbrli:measure></xbrli:unit><xbrli:unit id="pure"><xbrli:measure>xbrli:pure</xbrli:measure></xbrli:unit><xbrli:unit id="shares"><xbrli:measure>xbrli:shares</xbrli:measure></xbrli:unit><in-bse-co:ScripCode contextRef="MainI">532321</in-bse-co:ScripCode><in-bse-co:Symbol contextRef="MainI">ZYDUSLIFE</in-bse-co:Symbol><in-bse-co:MSEISymbol contextRef="MainI">NOTLISTED</in-bse-co:MSEISymbol><in-bse-co:NameOfTheCompany contextRef="MainI">ZYDUS LIFESCIENCES LIMITED</in-bse-co:NameOfTheCompany><in-bse-co:NameOfTheSignatory contextRef="MainI">DHAVAL N. SONI</in-bse-co:NameOfTheSignatory><in-bse-co:DesignationOfSignatory contextRef="MainI">Company Secretary and Compliance Officer</in-bse-co:DesignationOfSignatory><in-bse-co:Place contextRef="MainI">AHMEDABAD</in-bse-co:Place><in-bse-co:DateOfFiling contextRef="MainI">2026-08-24</in-bse-co:DateOfFiling><in-bse-co:ISINCode contextRef="MainI">INE010B01027</in-bse-co:ISINCode><in-bse-co:DisclosureUnderRegulation contextRef="MainI">Regulation 7 (2)</in-bse-co:DisclosureUnderRegulation><in-bse-co:RevisedFilling contextRef="MainI">false</in-bse-co:RevisedFilling><in-bse-co:TypeOfInstrument contextRef="Disclosure1">Equity</in-bse-co:TypeOfInstrument><in-bse-co:CategoryOfPerson contextRef="Disclosure1">Promoter Group</in-bse-co:CategoryOfPerson><in-bse-co:NameOfThePerson contextRef="Disclosure1">Prashant Babubhai Patel</in-bse-co:NameOfThePerson><in-bse-co:SecuritiesHeldPriorToAcquisitionOrDisposalNumberOfSecurity contextRef="Disclosure1" unitRef="shares" decimals="INF">0</in-bse-co:SecuritiesHeldPriorToAcquisitionOrDisposalNumberOfSecurity><in-bse-co:SecuritiesHeldPriorToAcquisitionOrDisposalPercentageOfShareholding contextRef="Disclosure1" unitRef="pure" decimals="INF">0</in-bse-co:SecuritiesHeldPriorToAcquisitionOrDisposalPercentageOfShareholding><in-bse-co:SecuritiesAcquiredOrDisposedNumberOfSecurity contextRef="Disclosure1" unitRef="shares" decimals="INF">32577</in-bse-co:SecuritiesAcquiredOrDisposedNumberOfSecurity><in-bse-co:SecuritiesAcquiredOrDisposedValueOfSecurity contextRef="Disclosure1" unitRef="INR" decimals="0">0</in-bse-co:SecuritiesAcquiredOrDisposedValueOfSecurity><in-bse-co:SecuritiesAcquiredOrDisposedTransactionType contextRef="Disclosure1">Buy</in-bse-co:SecuritiesAcquiredOrDisposedTransactionType><in-bse-co:SecuritiesHeldPostAcquistionOrDisposalNumberOfSecurity contextRef="Disclosure1" unitRef="shares" decimals="INF">32577</in-bse-co:SecuritiesHeldPostAcquistionOrDisposalNumberOfSecurity><in-bse-co:SecuritiesHeldPostAcquistionOrDisposalPercentageOfShareholding contextRef="Disclosure1" unitRef="pure" decimals="INF">0</in-bse-co:SecuritiesHeldPostAcquistionOrDisposalPercentageOfShareholding><in-bse-co:DateOfAllotmentAdviceOrAcquisitionOfSharesOrSaleOfSharesSpecifyFromDate contextRef="Disclosure1">2026-08-19</in-bse-co:DateOfAllotmentAdviceOrAcquisitionOfSharesOrSaleOfSharesSpecifyFromDate><in-bse-co:DateOfAllotmentAdviceOrAcquisitionOfSharesOrSaleOfSharesSpecifyToDate contextRef="Disclosure1">2026-08-19</in-bse-co:DateOfAllotmentAdviceOrAcquisitionOfSharesOrSaleOfSharesSpecifyToDate><in-bse-co:ModeOfAcquisitionOrDisposal contextRef="Disclosure1">Others</in-bse-co:ModeOfAcquisitionOrDisposal><in-bse-co:DateOfIntimationToCompany contextRef="Disclosure1">2026-08-21</in-bse-co:DateOfIntimationToCompany><in-bse-co:ExchangeOnWhichTheTradeWasExecuted contextRef="Disclosure1">NA</in-bse-co:ExchangeOnWhichTheTradeWasExecuted><in-bse-co:DetailsOfChangeInHoldingOfSecuritiesOfPromotersEmployeeOrDirectorOfAListedCompanyAndOtherSuchPersonsTextBlock contextRef="Disclosure1">The said transation is a tranmission of 32,577 equity shares from Late Jasodaben Babubhai Patel, Member of the Promoter Group of the Company. Since, there is no option to select "Transmission of shares" in transaction type, we have selected "Buy".</in-bse-co:DetailsOfChangeInHoldingOfSecuritiesOfPromotersEmployeeOrDirectorOfAListedCompanyAndOtherSuchPersonsTextBlock></xbrli:xbrl>
