<?xml version="1.0" encoding="UTF-8" standalone="no"?><!--ICGIG--><xbrli:xbrl xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:in-capmkt="http://www.sebi.gov.in/xbrl/2024-12-31/in-capmkt" xmlns:in-capmkt-ent="http://www.sebi.gov.in/xbrl/IntegratedGovernance/2024-12-31/in-capmkt/in-capmkt-ent" xmlns:in-capmkt-roles="http://www.sebi.gov.in/xbrl/IntegratedGovernance/2024-12-31/in-capmkt-roles" xmlns:in-capmkt-types="http://www.sebi.gov.in/xbrl/2024-12-31/in-capmkt-types" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:negated="http://www.xbrl.org/2009/role/negated" xmlns:net="http://www.xbrl.org/2009/role/net" xmlns:nonnum="http://www.xbrl.org/dtr/type/non-numeric" xmlns:num="http://www.xbrl.org/dtr/type/numeric" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:xl="http://www.xbrl.org/2003/XLink" xmlns:xlink="http://www.w3.org/1999/xlink"><link:schemaRef 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scheme="http://www.bseindia.com/in-capmkt/Symbol">MONOPHARMA</xbrli:identifier></xbrli:entity><xbrli:period><xbrli:instant>2025-06-30</xbrli:instant></xbrli:period><xbrli:scenario><xbrldi:typedMember dimension="in-capmkt:ImpositionOfFineOrPenaltyAxis"><in-capmkt:ImpositionOfFineOrPenaltyDomain>ImpositionOfFineOrPenaltyDomain1</in-capmkt:ImpositionOfFineOrPenaltyDomain></xbrldi:typedMember></xbrli:scenario></xbrli:context><xbrli:unit id="pure"><xbrli:measure>xbrli:pure</xbrli:measure></xbrli:unit><in-capmkt:ScripCode contextRef="MainI">000000</in-capmkt:ScripCode><in-capmkt:Symbol contextRef="MainI">MONOPHARMA</in-capmkt:Symbol><in-capmkt:MSEISymbol contextRef="MainI">NOTLISTED</in-capmkt:MSEISymbol><in-capmkt:ISIN contextRef="MainI">INE0OIP01012</in-capmkt:ISIN><in-capmkt:NameOfTheCompany contextRef="MainI">MONO PHARMACARE LIMITED</in-capmkt:NameOfTheCompany><in-capmkt:DateOfStartOfFinancialYear contextRef="MainI">2025-04-01</in-capmkt:DateOfStartOfFinancialYear><in-capmkt:DateOfEndOfFinancialYear contextRef="MainI">2026-03-31</in-capmkt:DateOfEndOfFinancialYear><in-capmkt:ReportingQuarter contextRef="MainI">Quarterly</in-capmkt:ReportingQuarter><in-capmkt:DateOfEndOfReportingPeriod contextRef="MainI">2025-06-30</in-capmkt:DateOfEndOfReportingPeriod><in-capmkt:TypeOfCompany contextRef="MainD">SME</in-capmkt:TypeOfCompany><in-capmkt:WhetherPartAOfAnnexureIOfTheSEBICircularDated31December2024RelatedToComplianceReportOnCorporateGovernanceIsApplicableToTheEntity contextRef="MainD">false</in-capmkt:WhetherPartAOfAnnexureIOfTheSEBICircularDated31December2024RelatedToComplianceReportOnCorporateGovernanceIsApplicableToTheEntity><in-capmkt:ReasonForPartAOfAnnexureIOfTheSEBICircularDated31December2024RelatedToComplianceReportOnCorporateGovernanceIsNotApplicableToTheEntity contextRef="MainD">As per regulation 15 of SEBI (Listing Obligations and Disclosure Requirements) Regulation, 2015, 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quarter.</in-capmkt:ReasonForPartEOfAnnexureIOfTheSEBICircularDated31December2024RelatedToDisclosureOfUpdatesToOngoingTaxLitigationsOrDisputesIsNotApplicableToTheEntity><in-capmkt:IsSCORESIDAvailable contextRef="MainI">true</in-capmkt:IsSCORESIDAvailable><in-capmkt:SCORESRegistrationID contextRef="MainI">comm01012</in-capmkt:SCORESRegistrationID><in-capmkt:TypeOfSubmission contextRef="MainI">Original</in-capmkt:TypeOfSubmission><in-capmkt:NoOfInvestorComplaints contextRef="PY_I" decimals="0" unitRef="pure">0</in-capmkt:NoOfInvestorComplaints><in-capmkt:NoOfInvestorComplaintsReceivedDuringThePeriod contextRef="MainD" decimals="0" unitRef="pure">0</in-capmkt:NoOfInvestorComplaintsReceivedDuringThePeriod><in-capmkt:NoOfInvestorComplaintsDisposedOffDuringThePeriod contextRef="MainD" decimals="0" unitRef="pure">0</in-capmkt:NoOfInvestorComplaintsDisposedOffDuringThePeriod><in-capmkt:NoOfInvestorComplaints contextRef="MainI" decimals="0" unitRef="pure">0</in-capmkt:NoOfInvestorComplaints><in-capmkt:NoOfInvestorComplaintsDuringThePeriod contextRef="MainD" decimals="0" unitRef="pure">0</in-capmkt:NoOfInvestorComplaintsDuringThePeriod><in-capmkt:AnyOtherInformationForDisclosureOfImpositionOfFineOrPenaltyExplanatoryTextBlock contextRef="MainD">company has already appointed the company secretary and compliance officer.</in-capmkt:AnyOtherInformationForDisclosureOfImpositionOfFineOrPenaltyExplanatoryTextBlock><in-capmkt:NameOfTheAuthority contextRef="I_ImpositionOfFineOrPenalty1">NATIONAL STOCK EXCHANGE (NSE)</in-capmkt:NameOfTheAuthority><in-capmkt:NatureAndDetailsOfTheActionTakenOrOrderPassed contextRef="I_ImpositionOfFineOrPenalty1">NOTICE OF FINE IMPOSED SENT BY NSE</in-capmkt:NatureAndDetailsOfTheActionTakenOrOrderPassed><in-capmkt:DateOfReceiptOfDirectionOrOrderIncludingAnyAdInterimOrInterimOrdersOrAnyOtherCommunicationFromTheAuthority contextRef="I_ImpositionOfFineOrPenalty1">2025-05-20</in-capmkt:DateOfReceiptOfDirectionOrOrderIncludingAnyAdInterimOrInterimOrdersOrAnyOtherCommunicationFromTheAuthority><in-capmkt:DetailsOfTheViolationOrContraventionCommittedOrAllegedToBeCommitted contextRef="I_ImpositionOfFineOrPenalty1">Delayed in appointment of company secretary and compliance officer .</in-capmkt:DetailsOfTheViolationOrContraventionCommittedOrAllegedToBeCommitted><in-capmkt:ImpactOnFinancialOrOperationOrOtherActivitiesOfTheListedEntityQuantifiableInMonetaryTermsToTheExtentPossible contextRef="I_ImpositionOfFineOrPenalty1">To the extent of fine imposed i.e Rs. 47,200/-.</in-capmkt:ImpactOnFinancialOrOperationOrOtherActivitiesOfTheListedEntityQuantifiableInMonetaryTermsToTheExtentPossible></xbrli:xbrl>