<?xml version="1.0" encoding="UTF-8"?>
<!--ANNAgreement V1.0 (10-04-2026)-->
<xbrli:xbrl xmlns:in-capmkt="https://www.sebi.gov.in/xbrl/2024-04-30/in-capmkt" xmlns:in-capmkt-ent="https://www.sebi.gov.in/xbrl/Announcement_For_Agreements/2024-04-30/in-capmkt/in-capmkt-ent" xmlns:in-capmkt-roles="https://www.sebi.gov.in/xbrl/Announcement_For_Agreements/2024-04-30/in-capmkt-roles" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:nonnum="http://www.xbrl.org/dtr/type/non-numeric" xmlns:in-capmkt-type="https://www.sebi.gov.in/xbrl/2024-04-30/in-capmkt-types" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:net="http://www.xbrl.org/2009/role/net" xmlns:num="http://www.xbrl.org/dtr/type/numeric" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:negated="http://www.xbrl.org/2009/role/negated" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:xl="http://www.xbrl.org/2003/XLink"><link:schemaRef xlink:type="simple" xlink:href="in-capmkt-ent-2024-04-30.xsd"/><xbrli:context id="MainD"><xbrli:entity><xbrli:identifier scheme="https://www.sebi.gov.in/in-capmkt/NSESymbol">THYROCARE</xbrli:identifier></xbrli:entity><xbrli:period><xbrli:startDate>2026-08-19</xbrli:startDate><xbrli:endDate>2026-08-19</xbrli:endDate></xbrli:period></xbrli:context><xbrli:context id="MainI"><xbrli:entity><xbrli:identifier scheme="https://www.sebi.gov.in/in-capmkt/NSESymbol">THYROCARE</xbrli:identifier></xbrli:entity><xbrli:period><xbrli:instant>2026-08-19</xbrli:instant></xbrli:period></xbrli:context><in-capmkt:NSESymbol contextRef="MainI">THYROCARE</in-capmkt:NSESymbol><in-capmkt:NameOfTheCompany contextRef="MainI">Thyrocare Technologies Limited</in-capmkt:NameOfTheCompany><in-capmkt:TypeOfAnnouncement contextRef="MainI">New</in-capmkt:TypeOfAnnouncement><in-capmkt:TypeOfAgreement contextRef="MainI">Others</in-capmkt:TypeOfAgreement><in-capmkt:DetailsOfOtherTypeOfAnnouncement contextRef="MainI">Transaction Documents</in-capmkt:DetailsOfOtherTypeOfAnnouncement><in-capmkt:StageOfAgreement contextRef="MainI">Termination of agreement</in-capmkt:StageOfAgreement><in-capmkt:AgreementsEnteredIntoBy contextRef="MainD">Promoters of the listed entity,Promoter Group entities of the listed entity</in-capmkt:AgreementsEnteredIntoBy><in-capmkt:WhetherEventOrInformationDisclosedIsAnOutcomeOfBoardMeeting contextRef="MainD">false</in-capmkt:WhetherEventOrInformationDisclosedIsAnOutcomeOfBoardMeeting><in-capmkt:DateOfOccurrenceOfEvent contextRef="MainI">2026-08-19</in-capmkt:DateOfOccurrenceOfEvent><in-capmkt:TimeOfOccurrenceOfEvent contextRef="MainD">16:47:00</in-capmkt:TimeOfOccurrenceOfEvent><in-capmkt:WhetherTheListedEntityIsPartyToTheAgreement contextRef="MainD">false</in-capmkt:WhetherTheListedEntityIsPartyToTheAgreement><in-capmkt:DateOfReport contextRef="MainI">2026-08-19</in-capmkt:DateOfReport><in-capmkt:DateOfInitialDisclosureOfTheExecutionOfTheAgreementToTheStockExchange contextRef="MainI">2025-09-15</in-capmkt:DateOfInitialDisclosureOfTheExecutionOfTheAgreementToTheStockExchange><in-capmkt:BriefDetailsOfTerminationOfAgreement contextRef="MainI">The NCDs issued by API, aggregating to INR 1,700 crore, were secured by pledge of equity shares representing 60.92% of the equity share capital of the Company held by Docon. Upon full redemption and repayment of the NCDs, the pledge has been fully released, and the Transaction Documents stand discharged and are no longer operative or effective.</in-capmkt:BriefDetailsOfTerminationOfAgreement><in-capmkt:NamesOfPartiesWithWhomTheAgreementWasEntered contextRef="MainI">1. Docon Technologies Private Limited
2. API Holdings Limited
3. AHWSPL India Private Limited
4. Catalyst Trusteeship Limited</in-capmkt:NamesOfPartiesWithWhomTheAgreementWasEntered><in-capmkt:NatureOfTheAgreementForTerminationOfAgreement contextRef="MainI">Transaction Documents (Debenture Trust Deed, Debenture Trustee Agreement, Deed of Hypothecation, Share Pledge Agreements and Deeds of Guarantee)</in-capmkt:NatureOfTheAgreementForTerminationOfAgreement><in-capmkt:DateOfExecutionOfTheAgreement contextRef="MainI">2025-09-11</in-capmkt:DateOfExecutionOfTheAgreement><in-capmkt:ReasonForTerminatingTheAgreement contextRef="MainI">Full redemption and repayment of the NCDs by API, resulting in the discharge of the related Transaction Documents and complete release of the pledge over the equity shares of the Company.</in-capmkt:ReasonForTerminatingTheAgreement><in-capmkt:DetailsOfImpactOfTerminationOfTheAgreement contextRef="MainI">No impact on the Company. The pledge over the equity shares has been fully released and all obligations under the Transaction Documents have been discharged</in-capmkt:DetailsOfImpactOfTerminationOfTheAgreement><in-capmkt:OtherInformationForTerminationOfTheAgreement contextRef="MainI">In the “Date of Execution of the Agreement” field, only the date can be entered. Accordingly, it may be noted that the date of execution of the agreement is September 11, 2025 (original execution date of the Transaction Documents), and the No Dues and Release Certificate was received from the Debenture Trustee on August 19, 2026.</in-capmkt:OtherInformationForTerminationOfTheAgreement></xbrli:xbrl>
